DOIT 3D Micro official launch approaching

Doitplenoptic will bring you very soon the first plenoptic ocular: DOIT 3D MICRO. This device is placed in the microscope eyepiece port and allows you to convert your microscope into a 3D microscope. No matter which brand you are using, if you need 3D information, you get it right away by inserting our plenoptic eyepiece!

We have a highly qualified R&D team and scientific advisory of Manuel Martínez-Corral and Genaro Saavedra, co-directors of the Image and 3D Display Laboratory of the University of Valencia. We have been working together since 2018th to develop the technology behind the eyepiece.

DOIT 3D Micro and Lightfield technology

Plenoptic photography, also known as integral imaging or lightfield imaging, consists of recording multiple perspectives of a 3D scene through an array of lenses. This way, the three-dimensional information is stored with single shotD allowing to register 3d images in real-time.DOIT® (Digital Optical Imaging Technology) is based on a paradigm shift in the capture of the plenoptic information. Conventional plenoptic systems capture low-resolution images close to the lens array; DOIT’s technology does it virtually at infinity with an improved lateral and axial resolution and higher depth of field.

Over this paradigm shift, DOIT® has developed a universal, versatile, affordable, and handy device: The DOIT 3D Micro eyepiece.

  • DOIT 3D Micro eyepiece can be used with any microscope,
  • Thanks to DOIT®, microscopists get seamless integration working with the objectives in the nosepiece,
  • Zero complexity, little investment, great results.

Stay attend to the launch of our device! DOIT 3D Micro will be available on the market very soon, and if you want to be one of the first users, do not hesitate to contact us.

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Miniscope3D: miniature fluorescence microscope

K. Yanny, N. Antipa, W. Liberti, S. Dehaeck, K. Monakhova, F. L. Liu, K. Shen, Ren Ng, and L. Waller, “Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy,” Light Sci Appl. 9, 171 (2020).

Under the leadership of Laura Waller, researchers from the University of California, Berkeley, have reported a lightfield miniscope that is much smaller and lighter than previous ones, and that provides with 3D images with unprecedent resolution over a very large depth of field.

The 3D miniscope is based in the smart combination of three bright ideas: the Fourier lightfield concept, the use of an optimized multifocal phase mask, and the application of a rendering algorithm based on sparsity-constrained inverse methods

Based on those concepts, authors have built a demonstration prototype composed basically by a GRIN-lens objective, a phase mask inserted at the Fourier plane and a CMOS sensor placed at the mean focal length of the phase mask. With this prototype, authors have demonstrated the capability of render 3D images of sparse fluorescent samples with lateral resolution of  across a depth of 2,76µm across a depth of 390µm.

In this paper the Miniscope3D demonstrates its utility providing 3D images of mouse brain tissue and also of freely moving tardigrades. In summary, the miniscope3D provides single-shot 3D imaging for applications where a compact platform matters, such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.

Commented by Dr. Manuel Martínez

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Plenoptic eyepiece. Transform any microscope into a 3D microscope

Doitplenotic will attend Focus on Microscopy 2021. Our scientific advisor Genaro Saavedra will present the paper titled “Plenoptic eyepiece. Transform any microscope into a 3D microscope”.

FOM2021 online will be the continuation of a yearly conference series on the latest innovations and developments in mostly optical microscopy and their application in biology, medicine, and the material sciences.

Key subjects for the conference series are the theory and practice of 3D optical imaging, related 3D image processing, and especially developments in resolution and imaging modalities. The conference series covers also the rapidly advancing fluorescence labeling techniques for confocal and multi-photon 3D imaging of -live- biological specimens.

Plenoptic eyepiece

Lightfield or plenoptic cameras are based on integral-photography concept and this can be applied to optical microscopy, but in the recent year a new architecture for lightfield microscopy has been proposed, it is named: Fourier lightfield, this scheme is based on collecting the spatio-angular field at the Fourier plane of the microscope [1].

The plenoptic eyepiece is based on Fourier lightfield, this is a portable, plug-and-play device that, after inserted at the ocular port, converts any conventional optical microscope into a lightfield microscope, with the best performance in terms of resolution and depth of field and allows you transform any microscope into a 3D microscope.

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Lightfield microscopy, a technique to study neural activities

Z. Zhang, L. Cong, L. Bai, and K. Wang, “Light-field microscopy for fast volumetric brain imaging,” Journal of Neuroscience Methods 352, 109083 (2021)

Researchers of the Center for Excellence in Brain Science and Intelligence Technology (Shanghai) have published this interesting paper in which a review of techniques for volumetric brain imaging is made.

Recording neural activities over large populations are critical for a better understanding of the functional mechanisms of animal brains. In this sense, the authors review different inspection techniques starting from those based on 3D scannings, like two-photon microscopy, which has the problem of low process speed and high light density. Another possibility, based on parallelizing the imaging process, is light-sheet microscopy which still has the problem requiring axial scanning.

Light-field-3d-microscopy for fast volumetric-brain-imaging

Confocal LFM (Zhang et al., 2020). MIPs over time of representative planes in reconstructed volumes in larval zebrafish brain (HuC: GCaMP6s). Scale bars, 50 μm.

In author’s opinion, lightfield microscopy (LFM) solves these problems elegantly by recording both the direction and location of light rays and achieving scanning-free and instantaneous volumetric imaging with a single camera exposure. However, this is made at the cost of a poor spatial resolution. But this drawback is overcome with the Fourier lightfield (FLFM) configuration, which shows substantially enhanced performance compared to that of conventional LFMs, including a lack of reconstruction artifacts near the focal plane, improved 3D reconstruction performance, and significantly reduced computational cost.

The paper finishes by collecting the results of LFM and FLFM when applied to brain images of different animals, like drosophila, zebrafish, or mouse. These results confirm the great utility of Fourier lightfield microscopy for brain imaging.

Commented by Dr. Manuel Martínez

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