Plenoptic photography, also known as integral technology or lightfield technology, consist of recording multiple perspectives of a sample or 3D scene using an array of lenses or microlenses that is placed somewhere between the scene and an image sensor. Thus, the 3D information, that is the information of the trajectories of the rays that come from the scene, is stored in an array of 2D microimages.
For 15 years, the implementation of lightfield microscopy (also called plenoptic or integral microscopy) has been proposed. Lightfield microscopy has the capacity to record the 3D information of thick samples without the need to perform more than a single shot. By capturing different perspectives and using the appropriate algorithms, it is possible to perform in-depth reconstruction (focusing on different planes) and calculate a depth map of the distinguishable parts across the width and length of the sample.